10 Types of Protein Powder: Nutrition, Amino Acids, and Bioavailability

We all have different protein requirements, and some of us opt to use protein powders to increase our daily intake.

However, there are many different types of protein powder available, and some offer more than others.

In this article, we take an in-depth look at nine of the most popular options alongside their nutrition facts, amino acid profiles, and protein quality scores.

Evaluating the Quality of Each Protein Powder

To assess the quality of each protein powder, we will look at three main factors;

  • Total protein: the amount of protein per serving.
  • Amino acid profiles: the concentrations of each amino acid.
  • Bioavailability: how bioavailable each protein powder is, as judged by modern protein scoring systems such as DIAAS (where possible) and PDCAAS.

For each protein powder, we will also provide full nutritional values per 30 grams, which represents a typical serving size.

All nutritional values come from the NCC Food and Nutrient Database.

Additional data on amino acid profiles and protein quality was attained from published research on commercially available plant proteins and protein quality studies.

What Are DIAAS and PDCAAS?

If anyone reading does not know what DIAAS and PDCAAS are, then here is a quick summary;

  • DIAAS (Digestible Indispensable Amino Acid Scores): the most accurate way to measure protein bioavailability. DIAAS measures the digestibility of each amino acid at the end of the small intestine (1).
  • PDCAAS (Protein Digestibility Corrected Amino Acid Score): this scoring system measures protein quality by analyzing the amino acid profile of the food. While useful, it does not factor anti-nutrients in food and how much protein our body can digest. For this reason, the FAO has proposed DIAAS as the optimal protein scoring system (2).

For both scores; the higher, the better. The maximum possible score for PDCAAS is 1.0, but DIAAS can go higher.

According to DIAAS, a rating of over 1.0 is a ‘high quality’ protein. Anything over 0.75 is medium quality, and anything below is low quality.

Let’s now take a look at some of the most common types of protein powder in alphabetical order;

1) Beef Protein Isolate

Beef Protein Isolate In a Measuring Scoop.

Beef protein isolate is a protein powder with a name that can easily confuse people.

From the name, some people may imagine dried and powdered steak.

However, beef protein isolate is just a flashier name for collagen, and it is primarily a source of glycine.

As a result, beef protein isolate is not a “complete” protein, and it does not contain meaningful amounts of the amino acids tryptophan and methionine (3).

Collagen can come from bones, connective tissues, and skin.

Nutrition and Amino Acid Profile

Nutrition Facts For Beef Protein Isolate (per 30 grams)
Calories/Nutrient Amount
Calories 96 kcal
Carbohydrate 0 g
Fat 0 g
Protein 24 g
Essential Amino Acids Amount
Histidine 0.70 g
Isoleucine 1.24 g
Leucine 2.62 g
Lysine 1.10 g
Methionine 0.82 g
Phenylalanine 1.65 g
Threonine 1.12 g
Tryptophan 0.33 g
Valine 1.66 g
Non-Essential Amino Acids Amount
Alanine 2.39 g
Arginine 2.27 g
Aspartic acid 1.55 g
Cysteine 0.01 g
Glutamic acid 3.15 g
Glycine 5.57 g
Proline 3.94 g
Serine
Tyrosine 0.18 g

Protein Quality

Since it does not contain adequate amounts of all essential amino acids, beef protein isolate does not do well on protein quality scores.

  • Total protein per serve: 28 grams
  • DIAAS score: 0 (low quality)
  • PDCAAS score: 0

According to PDCAAS and DIAAS scores, hydrolyzed collagen has a protein quality score of 0 (4).

Key Point: Beef protein isolate is not among the best protein powder options.

2) Brown Rice Protein Concentrate

Rice protein concentrate is a plant-based protein that is popular among vegetarians and those with dairy sensitivities.

Producers make this type of protein powder by treating rice with enzymes that separate the proteins from carbohydrates (5).

However, how does it compare to traditional dairy-based protein powders?

Let’s take a look.

Nutrition and Amino Acid Profile

Nutrition Facts For Rice Protein Concentrate (per 30g)
Calories/Nutrient Amount
Calories 90 kcal
Carbohydrate 0 g
Fat 0 g
Protein 24 g
Essential Amino Acids Amount
Histidine 0.70 g
Isoleucine 1.24 g
Leucine 2.62 g
Lysine 1.10 g
Methionine 0.82 g
Phenylalanine 1.65 g
Threonine 1.12 g
Tryptophan 0.33 g
Valine 1.66 g
Non-Essential Amino Acids Amount
Alanine 1.76 g
Arginine 2.50 g
Aspartic acid 2.54 g
Cysteine 0.53 g
Glutamic acid 5.54 g
Glycine 1.24 g
Proline 1.46 g
Serine 1.53 g
Tyrosine 1.69 g

Protein Quality

As shown, rice protein concentrate has a lower overall protein content than beef protein isolate.

However, it has a much better amino acid profile. The rate-limiting amino acid in brown rice protein is lysine (6).

  • Total protein per serve: 24 grams
  • DIAAS: 0.37 (low quality)
  • PDCAAS: 0.42

As shown by the protein quality scores, rice protein concentrate is not a high-quality protein powder (4).

Key Point: Rice protein is not among the best protein powders.

3) Egg Protein Powder

Egg white protein is another animal-based protein primarily made from dried egg whites.

The production process of egg protein powder involves spray drying, which is the same process used to make powdered milk products (7).

Nutrition and Amino Acid Profile

Nutrition Facts For Egg Protein (per 30g)
Calories/Nutrient Amount
Calories 96.3 kcal
Carbohydrate 0.2 g
Fat 0.03 g
Protein 24.0 g
Essential Amino Acids Amount
Histidine 0.55 g
Isoleucine 1.28 g
Leucine 2.00 g
Lysine 1.50 g
Methionine 0.89 g
Phenylalanine 1.39 g
Threonine 1.07 g
Tryptophan 0.39 g
Valine 1.64 g
Non-Essential Amino Acids Amount
Alanine 1.46 g
Arginine 1.37 g
Aspartic acid 2.42 g
Cysteine 0.64 g
Glutamic acid 3.10 g
Glycine 0.82 g
Proline 0.91 g
Serine 1.62 g
Tyrosine 0.96 g

Protein Quality

Egg whites offer a good quality source of protein.

Firstly, eggs contain all nine essential amino acids in sufficient amounts.

  • Total protein per serve: 24 grams
  • DIAAS: 1.13 (high quality)
  • PDCAAS: 1.00

Due to the amino acid profile of egg protein, it is a high-quality and bioavailable protein (4).

On the negative side, egg white protein powder is prone to clumping and does not mix well. It can also lead to side effects, such as bloating and nausea (8).

Key Point: Egg white is a good quality protein powder, but it has some drawbacks too.

4) Hemp Protein

Hemp Protein Powder In a Large Measuring Scoop.

Hemp protein powder comes from hemp seeds and combines two forms of protein; albumin and edestin (9).

Hemp is one of the newer proteins on the market, and it is suitable for vegans.

Nutrition and Amino Acid Profile

Nutrition Facts For Hemp Protein (per 30g)
Calories/Nutrient Amount
Calories 112.50 kcal
Carbohydrate 9.38 g
Fat 2.81 g
Protein 13.13 g
Essential Amino Acids Amount
Histidine 0.47 g
Isoleucine 0.66 g
Leucine 1.03 g
Lysine 0.75 g
Methionine 0.38 g
Phenylalanine 0.75 g
Threonine 0.56 g
Tryptophan 0.19 g
Valine 0.75 g
Non-Essential Amino Acids Amount
Cysteine 0.19 g
Tyrosine 0.56 g

Protein Quality

As shown in the nutritional values, hemp is not an isolated source of protein, and it also contains carbohydrate and fat.

For this reason, the total protein per serving is lower than most other protein powders.

  • Total protein per serve: 15.0 grams
  • DIAAS: N/A
  • PDCAAS: 0.63 – 0.66

The PDCAAS score for hemp protein ranges from 0.63 to 0.66, but there are no available DIAAS scores. The limiting amino acid in hemp protein is lysine (10).

Key Point: Hemp protein has a reasonable amino acid profile, but it is lower in total protein than other options.

5) Milk Protein Isolate

Milk protein isolate is a concentrated form of milk solids that contains both whey and casein proteins.

As a result, it contains the same types of protein as found in regular milk.

Nutrition and Amino Acid Profile

Nutrition Facts For Milk Protein Isolate (per 30g)
Calories/Nutrient Amount
Calories 104 kcal
Carbohydrate 0 g
Fat 0 g
Protein 26.1 g
Essential Amino Acids Amount
Histidine 1.20 g
Isoleucine 1.33 g
Leucine 2.58 g
Lysine 2.11 g
Methionine 0.78 g
Phenylalanine 1.28 g
Threonine 1.20 g
Tryptophan 0.42 g
Valine 1.70 g
Non-Essential Amino Acids Amount
Alanine 0.89 g
Arginine 1.10 g
Aspartic acid
Cysteine 0.21 g
Glutamic acid 5.50 g
Glycine 0.50 g
Proline 2.61 g
Serine 1.46 g
Tyrosine 1.38 g

Protein Quality

Similar to all dairy products, milk protein isolate is an excellent source of bioavailable amino acids;

  • Total protein per serve: 26.1 grams
  • DIAAS: 1.18 (high quality)
  • PDCAAS: 1.00
Key Point: Milk protein isolate is one of the highest quality protein powders.

6) Pea Protein Concentrate

Pea Protein Powder.

Pea protein is another of the more recently trendy protein powders, and it is also the key ingredient in Beyond Meat burgers.

The production process for this protein is quite complex, and it involves (11);

  • Drying the peas
  • Removing the outer fiber-based layers
  • Milling and then separating the proteins and carbohydrates
  • Using an isoelectric precipitation method to isolate the protein

Nutrition and Amino Acid Profile

Nutrition Facts For Pea Protein Concentrate (per 30g)
Calories/Nutrient Amount
Calories 111 kcal
Carbohydrate 0.60 g
Fat 1.65 g
Protein 24.0 g
Essential Amino Acids Amount
Histidine 0.45 g
Isoleucine 1.14 g
Leucine 1.98 g
Lysine 1.89 g
Methionine 0.18 g
Phenylalanine 1.20 g
Threonine 0.81 g
Tryptophan 0.18 g
Valine 1.51 g
Non-Essential Amino Acids Amount
Alanine 0.99 g
Arginine 1.86 g
Aspartic acid 2.58 g
Cysteine 0.30 g
Glutamic acid 3.60 g
Glycine 0.84 g
Proline 0.81 g
Serine 1.95 g
Tyrosine 0.96 g

Protein Quality

Pea protein is relatively low in methionine and cysteine.

However, the amino acid profile and bioavailability are reasonably good for a plant-based protein powder.

  • Total protein per serve: 24.0 grams
  • DIAAS: 0.82 (medium quality)
  • PDCAAS: 0.89
Key Point: Pea protein is one of the best vegan-friendly protein powders.

7) Skim Milk Protein Powder

Skim milk protein is another dairy-based protein powder that is primarily a source of casein.

Although not as common as whey protein, casein-based proteins are still a popular choice in the world of supplements.

One of the main benefits of casein is that it is a slow-digesting protein that steadily releases amino acids into the blood (12).

For this reason, many athletes/bodybuilders take a source of casein before bed.

Nutrition and Amino Acid Profile

Nutrition Facts For Skim Milk Protein (per 30g)
Calories/Nutrient Amount
Calories 110 kcal
Carbohydrate 0 g
Fat 0 g
Protein 26.0 g
Essential Amino Acids Amount
Histidine 0.75 g
Isoleucine 1.33 g
Leucine 2.36 g
Lysine 1.94 g
Methionine 0.70 g
Phenylalanine 1.26 g
Threonine 1.07 g
Tryptophan 0.33 g
Valine 1.73 g
Non-Essential Amino Acids Amount
Alanine 0.72 g
Arginine 0.89 g
Aspartic acid 1.70 g
Cysteine 0.09 g
Glutamic acid 5.20 g
Glycine 0.44 g
Proline 2.45 g
Serine 1.47 g
Tyrosine 1.35 g

Protein Quality

According to protein quality scores, skim milk protein has good bioavailability.

However, it doesn’t quite match up to other dairy protein powders such as whey and milk protein (13);

  • Total protein per serve: 26.0 grams
  • DIAAS: 1.05 (high quality)
  • PDCAAS: 1.0
Key Point: Skim milk protein has a good amino acid profile and it has high digestibility.

8) Soy Protein Isolate

When it comes to plant-based proteins, soy protein isolate is probably the highest quality.

With the first production starting in 1959, soy protein isolate has been under production for several decades now (14).

Nutrition and Amino Acid Profile

Nutrition Facts For Soy Protein Isolate (per 30g)
Calories/Nutrient Amount
Calories 114 kcal
Carbohydrate 1.20 g
Fat 0.30 g
Protein 27.0 g
Essential Amino Acids Amount
Histidine 0.78 g
Isoleucine 1.26 g
Leucine 2.31 g
Lysine 1.92 g
Methionine 0.39 g
Phenylalanine 1.62 g
Threonine 1.08 g
Tryptophan 0.30 g
Valine 1.35 g
Non-Essential Amino Acids Amount
Alanine 1.23 g
Arginine 2.31 g
Aspartic acid 3.60 g
Cysteine 0.39 g
Glutamic acid 6.12 g
Glycine 1.23 g
Proline 1.59 g
Serine 1.68 g
Tyrosine 1.23 g

Protein Quality

Soy protein scores best among the plant protein powders for amino acid bioavailability.

It is also very high in protein per serving.

  • Total protein per serve: 27.0 grams
  • DIAAS: 0.90 (medium quality)
  • PDCAAS: 0.98

See this complete guide to soy protein supplementation for more information.

Key Point: Soy protein isolate is probably the highest quality plant-based protein powder.

9) Whey Protein Concentrate

Scoop of Whey Protein Powder.

Whey protein concentrate is one of the most common types of protein powder, and it is a concentrated source of whey protein from milk.

However, whey concentrate is not a pure source of protein. It also contains small amounts of fat and carbohydrate.

Nutrition and Amino Acid Profile

Nutrition Facts For Whey Protein Concentrate (per 30g)
Calories/Nutrient Amount
Calories 120 kcal
Carbohydrate 3.76 g
Fat 0.92 g
Protein 22.8 g
Essential Amino Acids Amount
Histidine 0.45 g
Isoleucine 1.13 g
Leucine 2.17 g
Lysine 1.96 g
Methionine 0.43 g
Phenylalanine 0.75 g
Threonine 1.15 g
Tryptophan 0.47 g
Valine 1.13 g
Non-Essential Amino Acids Amount
Alanine 0.99 g
Arginine 0.64 g
Aspartic acid 2.23 g
Cysteine 0.41 g
Glutamic acid 4.07 g
Glycine 0.41 g
Proline 1.36 g
Serine 1.68 g
Tyrosine 0.58 g

Protein Quality

Whey protein concentrate is a high-quality protein powder, and it contains somewhere in the region of 75-85% protein by weight.

Whey concentrate contains all the essential amino acids in sufficient amounts, and it is also highly bioavailable.

Here are the protein quality values;

  • Total protein per serve: 22.8 grams
  • DIAAS: 1.07 (high quality)
  • PDCAAS: 1.0
Key Point: Whey protein concentrate is one of the best-quality protein powders.

10) Whey Protein Isolate

There is little difference between whey protein concentrate and whey isolate.

In short; isolate undergoes slightly more processing to fully “isolate” the protein.

Therefore, whey isolate contains more protein—around 85-90% by weight—and less carbohydrate and fat.

Nutrition and Amino Acid Profile

Nutrition Facts For Whey Protein Isolate (per 30g)
Calories/Nutrient Amount
Calories 118 kcal
Carbohydrate 0.54 g
Fat 0.54 g
Protein 26.8 g
Essential Amino Acids Amount
Histidine 0.45 g
Isoleucine 1.68 g
Leucine 2.91 g
Lysine 2.91 g
Methionine 0.60 g
Phenylalanine 0.87 g
Threonine 1.83 g
Tryptophan 0.48 g
Valine 1.38 g
Non-Essential Amino Acids Amount
Alanine 1.38 g
Arginine 0.63 g
Aspartic acid 2.91 g
Cysteine 0.78 g
Glutamic acid 5.13 g
Glycine 0.48 g
Proline 1.65 g
Serine 1.11 g
Tyrosine 0.81 g

Protein Quality

Whey protein isolate contains a large concentration of protein, and it has excellent digestibility.

  • Total protein per serve: 26.8 grams
  • DIAAS: 1.09 (high quality)
  • PDCAAS: 1.0
Key Point: Whey protein isolate offers a large and bioavailable source of protein.

Which Protein Powder Is the Best?

There are a lot of different things to consider when determining which protein is the best option.

Firstly, the table below shows the ranking of each protein powder in order of protein bioavailability;

Protein Powder Ranking By Bioavailability
Position Type of Protein Powder DIAAS PDCAAS Protein
#1 Milk Protein Isolate 1.18 1.0 26.1 g
#2 Egg Protein Powder 1.13 1.0 24.0 g
#3 Whey Protein Isolate 1.09 1.0 26.8 g
#4 Whey Protein Concentrate 1.07 1.0 22.8 g
#5 Skim Milk Protein 1.05 1.0 26.0 g
#6 Soy Protein Isolate 0.90 0.98 27.0 g
#7 Pea Protein 0.82 0.89 24.0 g
#8 Hemp Protein N/A 0.63-0.66 15.0 g
#9 Rice Protein Concentrate 0.37 0.42 24.0 g
#10 Beef Protein Isolate 0 0 24.0 g

As shown in the table, the best protein powder for bioavailability of amino acids is milk protein isolate.

However, there are some extra options also to consider.

Animal-Based vs. Plant-Based Protein Powders

Although we can get protein from animal and plant foods, animal-based proteins typically offer the most.

However, it is possible to get enough protein from any of these supplements.

For those who prefer to avoid animal foods, then soy protein isolate appears to be the best plant-based protein powder.

Digestion Speed

Milk protein isolate offers the best digestibility, but some people may prefer to select a protein based on digestion speed.

On this note, the fastest digestion and absorption rate is for whey protein, while casein is the slowest-digesting protein (15).

Key Point: Milk protein isolate appears to be the protein powder that offers the most. However, due to their differing characteristics, some individuals may prefer different options.

Final Thoughts

Whole foods are the ideal protein sources, but protein supplements can be a useful tool for people trying to increase intake.

There are many different types of protein powder available on the market.

Generally speaking, the best quality protein powders are dairy-based options like milk protein and whey.

However, all of these options may appeal to different people, and all can contribute to the total protein intake.

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Michael Joseph, MSc

Michael works as a nutrition educator in a community setting and holds a Master's Degree in Clinical Nutrition. He believes in providing reliable and objective nutritional information to allow informed decisions.

2 thoughts on “10 Types of Protein Powder: Nutrition, Amino Acids, and Bioavailability”

  1. Thanks for the information, I would recommend to anyone else doing this research to checkout the effect on your body with taking these different supplements specifically plant vs animals based. Peace and best of wishes on your journey to whole health.

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